Robert Carver | 11/29/2006 11:09:15 PM
ACCESSION NO: 0208743 SUBFILE: CRIS
PROJ NO: LAB93820 AGENCY: CSREES LA.B
PROJ TYPE: HATCH PROJ STATUS: NEW
START: 01 OCT 2006 TERM: 30 SEP 2011
INVESTIGATOR: Chen, Z.
PLANT PATHOLOGY & CROP PHYSIOL
LOUISIANA STATE UNIVERSITY
BATON ROUGE, LOUISIANA 70893
Host-Pathogen Interactions in the Soybean Rust and Other Major Disease Pathosystems in Soybean
NON-TECHNICAL SUMMARY: Soybean rust is caused primarily by Phakopsora pachyrhizi, which was first reported in Japan in 1902. Soybean rust is now an emerging disease in the continental US since its discovery in Louisiana in November, 2004. Soybean rust can reduce yields by as much as 80% depending on rust severity, time of initial host infection, and the environmental conditions. It was estimated that yield loses could exceed 10% in most of the United States, and up to 50% in the Mississippi Delta and southeastern states, where the environmental conditions are particularly conducive, and the pathogen is expected to become established. The purpose of the present proposal is to enhance soybean resistance to soybean rust through understanding of host-fungus interactions.
OBJECTIVES: 1. Investigate the survivability of Phakopsora pachyrhizi urediniospores under winter conditions to assess the potential of soybean rust disease in the coming season 2. Culture P. pachyrhizi in vitro to understand nutritional, biochemical and physical requirement for fungal infection 3. Identify host and fungal proteins induced during infection and proteins differentially expressed between soybean lines (moderately) resistant and susceptible to soybean rust or other diseases using proteomics 4. Characterize these proteins, especially fungal hydrolytic enzymes and host resistance-related proteins, to understand host-fungus interactions and host resistance mechanisms 5. Verify the importance of promising host proteins in disease resistance through RNAi gene silencing. The long term objective is to develop new strategies to control soybean rust and other diseases of soybeans.
APPROACH: 1. Examine Phakopsora pachyrhizi spores germination rate under different temperature and humidity conditions; 2. Culture the rust fungus on detached soybean leaves, on medium containing soybean leaf extracts or on defined medium with different pore-size membranes; 3. Extract, separate soybean leaf proteins using proteomics, and compare for protein differences between infected and non infected samples, and between soybean lines (moderately) resistant and susceptible to soybean rust; 4. Examine the biological functions of these proteins through gene cloning, enzyme or antifungal activity assays; 5. Use RNAi gene silencing to verify the importance of identified proteins in disease resistance.
Name: Chen, Z. Y.