The LSU AgCenter Biotechnology Laboratory (ABL) is a core facility that provides basic and applied research expertise to support researchers in the LSU system as well as those in other academic institutions and industry. The ABL was formed in 1998 to provide support to faculty in performing biomolecular research. In 2010, the ABL was merged with the Protein Facility in LSU’s College of Basic Sciences. Today, the ABL consists of three units: the Protein Facility, the Plant Transformation Facility and the Animal Cell Culture Facility. The ABL can provide a wide range of services to investigators, often at a fraction of the price of commercial companies.
Dr. Ted Gauthier, Director
115 H.D. Wilson Laboratories
Baton Rouge, LA 70803
Phone: (225) 578-5230
Fax: (225) 578-7863
The Protein Facility is located in 104 H.D. Wilson Laboratories. The facility offers large- and small-scale peptide synthesis, preparative and analytical-scale HPLC, protein expression, circular dichroism, SDS/PAGE, 2-D electrophoresis, blotting, isoelectric focusing and digital image acquisition and analysis. Total genomic RNA or DNA extraction and purification from different organisms can be performed. DNA sequencing is available. Small- or large-scale plasmid DNA preparation and purification from E. coli or Agrobacterium is available.
Contact information: Dr. Ted Gauthier
Plant Transformation FacilityThe Plant Transformation Facility is located in 105 H.D. Wilson Laboratories. This state-of-the-art facility provides researchers with the infrastructure and expertise in the implementation of molecular biology and genetic engineering technologies in the areas of plant transformation and plant tissue culture. The facility provides multiple research groups centralized access to equipment and instrumentation used in direct and bacterial methods of DNA transmission into plant species of economic interest in Louisiana.Contact information: Dr. Ted Gauthier
Animal Cell Culture
The Animal Cell Culture lab is located in 142 H.D. Wilson Laboratories. This facility provides tissue culture to various units on campus. It contains equipment and all materials for the storage and maintenance of animal and human cell lines. The LN2 cell storage unit contains over 100 cell lines, and we regularly order new cell lines from ATCC as needed. The researchers that it supplies are testing bioactive compounds and extracts from plants and common foodstuffs to determine various cellular effects in vitro, including nutritional, anti-inflammatory and anti-cancer activities. The cells provided from this lab are also being used for DNA and RNA extraction and to create diagnostic tools. While we strive to keep our lab pathogen free, we provide cells for the growth of specific pathogens in other labs on campus. The lab has the capacity to produce hybridomas and primary cell cultures, if required. The unit frees students and other investigators from the time-consuming task of maintaining reliable, contaminant-free cell cultures as well as eliminating the need for Biosafety level 2 hoods, incubators and LN2 storage tanks to be maintained in numerous labs throughout campus. We are flexible and always adapting to new challenges in providing human and animal cells for research.
Contact information: Ms. Karen McDonough
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